Cobalamin Deficiency During Pregnancy Expressed as Elevated Urine Methylmalonic Acid Levels Determined by a Photometric Assay
نویسنده
چکیده
Aim: Deficiency of cobalamin and folate during pregnancy is associated with megaloblastic anemia. Lower levels of folate and vitamin B12 were reported in mothers whose offspring had neural tube defects compared to unaffected controls. Increased methylmalonic acid (MMA) levels are a sensitive indicator of mild vitamin B12 deficiency and elevated homocysteine levels denote vitamin B12 or folate deficiency. We aimed to evaluate cobalamin state in pregnancy by using urine MMA levels determined by an economic spectrophotometric method.
منابع مشابه
Urine methyl malonic acid levels in HIV-infected adults with peripheral neuropathy
Background: Cobalamin deficiency and peripheral neuropathy (PN) are commonly seen in HIV-infected adults. The level of urine methylmalonic acid (UMMA), a reliable indicator of tissue cobalamin status, was determined in HIV infected subjects with and without PN to establish this association. Methods: One hundred and ninety-eight (198) consenting HIV infected subjects with and without PN were rec...
متن کاملThe clinical evaluation of cobalamin deficiency by determination of methylmalonic acid in serum or urine is not invalidated by the presence of heterozygous methylmalonic-acidaemia.
It is well established that accumulation of methylmalonic acid may provide an early clue to the existence of tissue cobalamin (vitamin B12) deficiency. To verify whether methylmalonic acid accumulates in adult heterozygotes for inherited methylmalonic-acidaemia and thereby gives "false" positive test results for cobalamin deficiency, we measured the concentration of methylmalonic acid in serum ...
متن کاملUrine methylmalonic acid levels in HIV-infected adults with peripheral neuropathy.
BACKGROUND Cobalamin deficiency and peripheral neuropathy (PN) are commonly seen in HIV-infected adults. The level of urine methylmalonic acid (UMMA), a reliable indicator of tissue cobalamin status, was determined in HIV infected subjects with and without PN to establish this association. METHODS One hundred and ninety-eight (198) consenting HIV infected subjects with and without PN were rec...
متن کاملSolid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method.
Methylmalonic acid concentrations are increased in serum in vitamin B12 (cobalamin) deficiency. Here I demonstrate the successful use of anion-exchange extraction for improving newly developed analytical procedures and describe well-documented, reliable performance of this method for rapid determination of methylmalonic acid. The sorbent counter ion is formate, and the elution solvent is formic...
متن کاملCobalamin (vitamin B12) deficiency detection by urinary methylmalonic acid quantitation.
A study was made to assess the value of cobalamin deficiency detection through quantitation of urinary methylmalonic acid (MMA). Urinary MMA was measured in 1118 patients suffering from megaloblastic anemia, other anemias, elevated red cell mean corpuscular volume, or unexplained neurologic disorders. Patients without proven cobalamin deficiency had urinary MMA levels less than 20 micrograms/ml...
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